摘要
孟庆国,张铁垣.蛋白氨基酸衍生化条件的正交试验.数理统计与管理,1998,17(3),4~6.蛋白氨基酸经酯化和酰化两步衍生化之后,才能进行气相色谱分析,影响衍生化程度的因素主要是酯化时间、酯化温度、酰化时间和酰化温度。用正交试验研究了上述四因素对氨基酸衍生化程度的影响,正交表采用L9(34),使实验次数由传统实验的81次减少为9次,实验指标为相对重量校正因子S′W(内标物为鸟氨酸)。实验结果表明:对精氨酸、色氨酸和蛋氨酸来说,酰化时间的影响最大,酰化时间越长,对精氨酸和色氨酸的衍生化越有利,而蛋氨酸则正好相反,另外三因素对上述三种氨基酸衍生物的S′W影响较小。其余16种氨基酸的S′W在9次实验中变化不大。
he protein amino acids must be derivated before GC analysis and the derivation reagents are butanol and trifluoroacetic anhydride. The factors which will influence the extent of derivation of amino acids are esterification time, esterification temperature,acylation time and acylation temperature. In order to study the influence upon the extent of derivation of these factors above we adopted the orthogonal test L9(34). The result of the experiment shows that the acylation time is very important to the extent of derivation of Arg.、Trp. and Met..Arg.and Trp. need long acylation time and Met. is just the contrary. The S′W of other sixteen amino acids in all of the experiments vary little.
出处
《数理统计与管理》
CSSCI
北大核心
1998年第3期4-6,共3页
Journal of Applied Statistics and Management
关键词
正交实验
氨基酸
衍生化
醌化
酰化
蛋白质
orthogonal test, S′W(RWR),protein amino acids, derivation