应用原位杂交法对比研究慢型克山病、扩张型心肌病与肠道病毒的关系

A COMPARATIVE STUDY ON THE RELATIONSHIP BETWEEN CHRONIC KESHAN DISEASE AND DILATED CARDIOMYOPATHY AND ENTEROVIRUSES IN SITU HYBRIDIZATION

本文用生物素标记的柯萨奇B_3病毒(CVB_3)的cDNA探针,通过原位杂交技术检测50例组织学诊断为慢型克山病、14例扩张型心肌病的心肌石蜡包埋切片,并以15例正常心肌标本作阴性对照。结果50例慢型克山病有34例出现阳性结果。14例扩张型心肌病有7例出现阳性结果。15例正常对照心肌标本杂交均为阴性,从而确定慢型克山病的心肌组织中、扩张型心肌病的心肌组织中有肠道病毒核酸存在,表明肠道病毒感染与慢型克山病、扩张型心肌病的发病高度相关。经统计学处理二者之间无明显差异。

Coxsackievirus B3(CVB3)cDNA fragments were purified from E.coli HB101 containing the CVB3 recombinant plasmid(pGP51B). The targets fragments were labelled with Bio-11-dUTP by nick translation procedure. We performed in situ hybridization to examine RNA of CVB in paraffin-embedded myocardial specimens from 50 patients with chronic Keshan disease(CKD), 14 patients with dilated cardiomyopathy (DCM) and 15 normal myocardial specimens. The results showed the positive hybridization signals were observed in 34 of 50 patients with CKD, 7 of 14 patients with DCM and the negative signals in normal myocardial specimens. It suggested that the persistent presence of enterovirus infection probably was related with the pathogenesis of CKD and DCM.