高三尖杉酯碱抗乳腺癌作用的初步研究

A Study of Homoharringtonine in Anti-Breast Cancer

目的:研究药物高三尖杉酯碱是否对人乳腺癌MDA-MB-453细胞具有生长抑制和诱导凋亡作用,以挖掘研发治疗乳腺癌的新药物。方法:常规培养人乳腺癌MDA-MB-453细胞,根据高三尖杉酯碱浓度分为空白组和高三尖杉酯碱10、20、40、60、80、120、160μg/mL组,其中20μg/mL组为小剂量组,80μg/mL组为中剂量组,120μg/mL组为大剂量组。采用台盼蓝染色、MTT比色法和透射电镜分析研究高三尖杉酯碱对于人乳腺癌细胞的作用。结果:台盼蓝染色结果显示大、中、小剂量组的活细胞率依次由小到大;MTT比色法显示各浓度药物作用在24、48和72 h均能显著抑制人乳腺癌MDA-MB-453细胞的增殖(P<0.05,P<0.01)。电镜观察发现,高三尖杉酯碱作用后的人乳腺癌MDA-MB-453细胞染色体断裂,核分裂成碎片,细胞内形成凋亡小体。结论:高三尖杉酯碱对于乳腺癌MDA-MB-453细胞具有一定的抑制作用和诱导凋亡的作用,应予进一步研究。

Objective：To investigate the effect of homoharringtionine in growth inhibiting mechanisms and apoptosis induction of breast cancer cell MDA-MB-453 in humans in an effort to develop a new drug therapy of the breast cancer. Methods： Human breast cancer cell MDA-MB--453 was conventionally cultured. According to the concentration level,the cells were divided into blank group and homoharringtonine groups of 10,20,40,60,80,120 and 160 ug/mL. 20 ug/mL group was small dose group,80ug/mL group was medium dose group and 120 ug/mL was large dose group. The effect of homoharringtonine was analyzed by trypan blue staining,MTT chromatometry and transmission electronic microscope. Results ：The results of trypan blue staining showed that the living cell rates of the 3 groups were from low to high ; MTT method showed that different levels of concentration of the drug significantly inhibited the proliferation of the MDA-MB453 cell（ P 〈 0.05 ,P 〈 0. 01 ） in 24 h,48 h and 72 h, but the findings of the electronic microscope showed chromosome breakage in MDA-MB-453 cell,caryomitotic fragmentation and apoptotic bodies in cells after administraion of homoharringtonine. Conclusion ： Homoharringtonine can inhibit breast cancer cell MDA-MB-453 and induce apoptosis to some extent,but further research is needed.