系统性红斑狼疮患者外周血单一核细胞CX3CR1趋化因子受体mRNA的表达

Expression of chemokine receptors CX3CR1 mRNA in patients with systemic lupus erythematosus

目的:探讨趋化因子受体CX3CR1在系统性红斑狼疮(SLE)患者外周血单一核细胞(PBMC)中的表达,及其与疾病活动的相关性。方法:收集93例确诊的SLE患者和30例正常人的PBMC,提取RNA。应用RT-PCR方法检测CX3CR1mRNA表达水平,并与疾病活动度评分(SLEDAI)作直线相关性分析。结果:①CX3CR1 mRNA在PBMC中的表达水平,活动期(3.735±0.557)与非活动期(0.530±0.045),两者差异有统计学意义(t=2.606,P=0.01);非活动期与对照组(0.146±0.02)间的差异有统计学意义(t=5.831,P=0.000);患者组(2.819±1.347)与对照组间的差异有统计学意义(t=3.921,P=0.000)。②SLE患者组CX3CR1 mRNA水平与疾病活动度评分(SLEDAI)呈正相关(r=0.445,t=4.523,P<0.001)。③不具有血管炎、肌炎、肾损及血小板减少的患者与具有上述相应临床特征的患者比较,CX3CR mRNA表达水平更高,差异具有统计学意义。结论:CX3CR1mRNA表达水平在活动期SLE比非活动期增高,与疾病的活动性(SLEDAI)呈正相关。CX3CR1可能反映疾病的活动性、参与特异器官受累的发生。

Objective： To assess the expressions of chemokine receptors CX3CR mRNA on peripheral blood mononuclear cell（PBMC） with active or inactive systemic lupus erythematosus（SLE）.Methods： Ninty three definitive SLE patients and 30 controls were collected.PBMC were separated from the peripheral blood specimen.Reverse transcription-polymerase chain reaction（RT-PCR） technique was applied to the semi-quantitatively analyze for the expression of chemokine receptors mRNA（CX3CR1） in PBMC. Results： Level of CX3CR1 mRNA expression was significantly increased in patients with SLE（2.819±1.347）as compared with the controls（0.146±0.02）（t=3.921,P=0.000）.Level of CX3CR1 mRNA expression in PBMC showed significantly positive correlation with disease activity（SLEDAI）. Conclusion： CX3CR1 might be involved in the pathogenesis of SLE,and the expression level might be a good indicator for the disease activity of SLE.