选择素单抗对裸鼠胃癌转移抑制的研究

Inhibition of Human Stomach Carcinoma Metastasis in Vivo by Anti-P-Selectin Monoclonal Antibody

晚近发现的粘附分子P-选择素.能介导血小板和内皮细胞与肿瘤细胞之间相互作用,在肿瘤转移过程中起粘附介导作用。该文用人胃癌细胞株原位移植方法建立动物模型.术后分别注射P-选择素单克隆抗体或PBS液体。结果发现.PBS对照组肿瘤转移率为54.5%。单抗组仅1例有肿瘤转移。ELISA法测定显示,肿瘤转移裸鼠血浆P-选择素含量显著高于非转移裸鼠。表明P-选择素与肿瘤转移相关,其单抗对肿瘤转移有抑制作用。

P-selectin was found to contribute to the interaction of tumor cells with platelets and vascular endothelium. There have been so far no reports about human tumor metastasis blocked in vivo by selectin monoclonal antibodies (Mabs). We investigated the role of P-selectin Mabs in tumor metastasis inhibition using human gastric carcinoma cell line SGC- 7901. SGC - 7901 developed tumors in the stomach wall when injected orthotopically in BALB-c nude mice. Approximately one half of BALB- c nude mice receiving injections of PBS developed metastatic tumor, whereas only one of BALB-c nude mice receiving injections of P-selectin Mabs 3 days after tumor injection was found tumor metastasis. The plasma level of P-selectin in BALB-c nude mice with tumor metastasis, was higher than that withour such metastasis. The result suggests that P-selectin plays an important role in metastasis, and the metastasis can be blocked in vivo by the Mabs.