顶空气相色谱连续监测鼠伤寒沙门氏菌致突变试验研究

Study of Salmonella Mutagenicity Assay with Headspace Gas Chromatography by Monitoring Carbon Dioxide

在密闭的液体培养体系中，以ＣＯ２为指标用气相色谱分析法连续监测致突变剂ＮａＮ３和敌克松对鼠伤寒沙门氏菌组氨酸缺陷型菌株ＴＡ１００、ＴＡ９８的致突变作用，研究了回复突变菌数、致突变剂的剂量与ＣＯ２产生量之间的关系，在此基础上提出了致突变性的评价指标。用本方法测得ＮａＮ３和敌克松的最低诱变剂量分别为０．０５０μｇ／瓶和０．０８０μｇ／瓶，灵敏度显著高于Ａｍｅｓ试验。

For the preliminary screening of mutagents,a novel method was developed combining Salmonella mutagenicity assay with Headspace Gas Chromatography.By monitoring carbon dioxide with gas chromatography,we studied the effect of number of revertants and dose of mutagens on the output of CO 2.An index was selected to assess mutagenicity doseresponse relationship.For sodium azide and dexon,the minimum measurable mutagenesis dose were 0050 and 0080μg/bottle,respectively.