摘要
在密闭的液体培养体系中,以CO2为指标用气相色谱分析法连续监测致突变剂NaN3和敌克松对鼠伤寒沙门氏菌组氨酸缺陷型菌株TA100、TA98的致突变作用,研究了回复突变菌数、致突变剂的剂量与CO2产生量之间的关系,在此基础上提出了致突变性的评价指标。用本方法测得NaN3和敌克松的最低诱变剂量分别为0.050μg/瓶和0.080μg/瓶,灵敏度显著高于Ames试验。
For the preliminary screening of mutagents,a novel method was developed combining Salmonella mutagenicity assay with Headspace Gas Chromatography.By monitoring carbon dioxide with gas chromatography,we studied the effect of number of revertants and dose of mutagens on the output of CO 2.An index was selected to assess mutagenicity doseresponse relationship.For sodium azide and dexon,the minimum measurable mutagenesis dose were 0050 and 0080μg/bottle,respectively.
出处
《中国公共卫生》
CAS
CSCD
北大核心
1998年第1期45-46,共2页
Chinese Journal of Public Health
基金
国家自然科学基金
关键词
伤寒沙门氏菌
致突变试验
气相色谱法
Salmonella Mutagenicity Assay\ Liquid Medium\ Headspace Gas Chromatography\ Carbon Dioxide