聚合酶链反应结合地高辛标记探针杂交检测宫颈组织癌人乳头瘤病毒

Detect Human Papillomavirus in Cervical Cancer by Using Polymeras Chain Reaction Ligated with Digoxigenin Labeled Probe Hydrization

应用聚合酶链反应结合地高辛标记探针杂交方法检测宫颈癌组织中人乳头瘤病毒（ＨＰＶ）。应用１对通用型引物检测３６例新鲜活检宫颈癌组织中ＨＰＶ，凝胶电泳分析与聚合酶链反应结合印迹杂交（ＳｏｕｔｈｅｒｎＢｌｏｔ）及聚合酶链反应结合斑点杂交的检测阳性率分别为６６７％、６９４％、６９４％，结果基本一致。应用３对通用型引物检测宫颈癌组织中ＨＰＶ，ＨＰＶ的检测阳性率分别为７２２％（２６／３６）、７５０％（２７／３６）、６３２％（１２／１９），总的检测阳性率为９１７％（３３／３６）；在β球蛋白基因阳性的２７例宫颈癌组织中，总的检测阳性率为９００％（２４／２７）。说明聚合酶链反应结合地高辛探针检测ＨＰＶ方法可应用于宫颈癌的分子流行病学研究。

o explore and effective method to detect human papillomavirus(HPV)in cervial cancer.Polymerase chain reaction(PCR)ligated with digoxigenin labled probe hydridization was used to detect human papillomavirus in cervical cancer.Digoxigenin labeled probe could be used to determine the polymerase chain reaction product of human papillomavirus.The positive rate of human papillomavirus in 36 case of cervical cancer was 667%,694%,694% respectively by using one set of consensus primer through agarose gel electrophoresis,PCR ligated with Southern blot hybridization and PCR ligated with dot blot hybridization.The positive rate of human papillomavirus was 722%(26/36),750%(27/36),632%(12/19),(P<005),respectively by using three different sets of consensus primer,the total positive rate was 917%(33/36).The total positive rate of human papillomavirus in 27 cases of cervical cancer with βGlobin gene was 900%(24.27).The method to detect human papillomavirus by using polymerase chain reaction ligated with digoxigenin labled probed could be applied to the molecular epidemiology study of cervical cancer.The positive rate might be influenced by using different sets of consensus primer.