喉癌和癌旁组织P_(53)蛋白的表达及临床意义

P53 protein expression in laryngeal carcinoma and adjancent mucosa and its clinical significance

目的：探讨喉癌向周围组织浸润发展的方式。方法：应用抗癌基因Ｐ５３单克隆抗体（ＤＯ－１）对１９例喉癌、癌旁和相对正常组织进行免疫组织化学染色。结果：Ｐ５３蛋白阳性物质位于肿瘤细胞核和转化的细胞核内。喉癌的表达率为７３．６８％。癌旁的表达率为３６．８４％，且３～５个阳性细胞位于上皮基底部的细胞核内，呈局灶性、跳跃性表达。结论：喉癌细胞向周围组织浸润方式是跳跃性的，因此为了达到手术治愈目的，喉癌切除术的安全缘至少１ｃｍ。

Objective:To discuss the pattern of infiltration of laryngeal carcinoma.Methods:We analyzed immunohistochemically the P53 protein in laryngeal squamous cell carcinoma,adjacent mucosa to carcinoma and related normal mucosa in the same patients.Results:The P53 protein antigenicity was generally located in the nuclei of tumor cells and transformed cells.Fourteen of 19 cases of carcinoma showed P53 protein expression (73.68%);seven of 19 cases of adjancent mucosa to carcinoma showed the P53 protein expression (36.84%),in which three to five positive cells arranged in masses and jumped intervally in the basal part of epithelia;all the related normal mucosa showed P53 protein negative.Conclusion: These findings indicated that the pattern of tumor cell′s invasion is jumping,tumor cells jump out from their parent carcinoma nests towards the head where they grow rapidly,then meet their parents.So the safety region of laryngeal carcinoma must,at least, be one cm far from the edge of carcinoma for the purpose of operative treatment. 