龙胆草斑枯病菌生物学特性的研究

Biological Characteristics of Gentiana Spot Blotch Pathogen

通过不同培养处理，对龙胆草斑枯病菌生物学特性进行了试验研究。结果表明：病菌在８℃～３０℃范围内能够生长发育，但最适温度是２５℃。病原菌对营养要求较严格，在ＰＤＡ＋寄主煎汁培养基上生长发育最好。对Ｃ源的利用上，单糖好于双糖，双糖好于多糖。该病菌属喜铵态氮的真菌，病菌产孢需要光照，但光照对生长影响不明显。病菌分生孢子在温度５℃～３２℃，ｐＨ４５～７３，相对湿度９６％以上条件下都可以萌发，但在温度２５℃、ｐＨ６２、水中有空气条件下。

The results showed that the pathogen can grow and develop form 8℃ to 30℃, but the optimum growth temperature is 25℃; the nutritions are more important to pathogen, it developes the best on the medium PDA+decoct host; the monosaccharides are more than the disaccharides, the disaccharides are more than the polysaccharides; the pathogen belonges to like using the ammonia nitrogen. Forming spores need the light, but the affect of the light is not obvious to pathogen growth; The conidia can germinate between 5℃～32℃, pH 45～7.3 and above RH 96%; but the germination rate the hightes when temperature is 25℃, pH 6.2 and there is air.