肝素寡糖的制备及其对平滑肌细胞抗增生作用的初步研究

Preparation of Heparin-Derived Oligosaccharides and Effection of them on Vascular Smooth Muscle Cells Proliferation

制备肝素寡糖并初步研究其对平滑肌细胞增生的作用。采用Bio-GelP6凝胶色谱分离纯化低分子肝素,制备不同Mr的肝素寡糖并比较其对平滑肌细胞增生的作用。结果表明,分离得到的平均Mr约3200的肝素寡糖,具有明显抑制平滑肌细胞增生的作用,其抗FXa效价为48·90ant-FXaIU/ml。

Prepared heparin-derived oligosaceharides and inspect effections of them on vascular smooth muscle cells（VSMCs） proliferation, separated low molecular weight heparin（LMWH） by Bio-Gel P6 gel filled chromatographic columns. Prepared different size oligosaccharides and inspect their effection on VSMCs proliferation. The results indicated that one product showed remarkable antiproliferative effects on VSMCs. Anti-FXa activity of this product was 48. 90 anti-FXaIU/mg and its Mr was 3200.