摘要
目的建立含玻璃酸钠(SH)的复方硫酸软骨素滴眼液中硫酸软骨素(CS)的含量测定方法。方法通过醋酸钠调节样品的酸碱度和离子强度,氯化十六烷基吡啶(CPC)可选择性地沉淀CS,采用咔唑法测定CS的含量,而不受SH的影响。结果优化的样品预处理条件为:醋酸钠的浓度为16%,CPC的浓度为0.8%,充分搅拌后,静置2 h,20μm滤膜过滤;滤饼经8%氯化钠溶液超声解离后,用于CS的含量测定,回收率为100.2%,RSD为1.17%。结论此法简便,快速,结果准确,可作为含SH的复方硫酸软骨素滴眼液中CS的含量测定方法。
Purpose To establish a method tor determining the content of chondroitin sulfate(CS)in compound chondroitin sulfate eye drops containing sodium hyaluronate (SH). Methods A carbazole method was used for determining the content of CS. By regulating acidity or alkalinity and ionic strength with sodium acetate, CS was selectively precipitated with cetylpyridinium chloride(CPC), whereas SH was not. Results Optimal samples pretreatment conditions were as follows:The mass concentrations of sodium acetate and CPC were 16% and 0.8%, respectively, and they were stirred thoroughly, standing for 2 h, and filtered by 20μm film. The method recovery was 100.2%with RSD of 1.17%. Conclusion The method is simple, rapid and accurate. It can be used for determining the content of CS in compound ehondroitin sulfate eye drops containing SH.
出处
《中国生化药物杂志》
CAS
CSCD
北大核心
2009年第1期43-45,共3页
Chinese Journal of Biochemical Pharmaceutics
关键词
复方硫酸软骨素滴眼液
玻璃酸钠
咔唑法
含量测定
compound ehondroitin sulfate eye drops
sodium hyaluronate
carbazole method
determination