肝素钠对伪狂犬病毒侵染BHK-21细胞和小白鼠的抑制

THE EFFECT OF HEPARIN ON THE INFECTION OF PSEUDORABIES VIRUS(PRV) TO BHK_21 CELLS AND MOUSE

应用空斑技术，检测肝素钠对伪狂犬病毒（ＰＲＶ）在ＢＨＫ＿２１细胞上形成空斑的数量，证实肝素钠对ＰＲＶ的感染能力有较大抑制作用。小鼠试验证实肝素钠可降低病毒的致病力，但肝素钠对小鼠有一定毒性。

To determine the effect of heparin on the infection of PRV to BHK_21 cells and mouse,the number of plaques formed and protection of mouse from PRV challenge were investigated.At concentration of 0.01μg/ml,heparin had an inhibitory effect on the infection of PRV to BHK_21 cells.The higher the concentration,the stronger inhibition potency was manifested.When the concentration was 10μg/ml,the inhibition rate reached 97.4%.Likewise,heparin could inhibit the infection of PRV to mouse.The suitable concentration was 5μg/ml.While 10μg/ml at a dose of 0.2ml was lethal to mouse.