DNA银纳米线的制备及其拉曼光谱

Preparation of DNA Silver Nanowire and Its Raman Spectra

根据DNA分子的静电自组装性质,利用加热和紫外照射相结合的方法制备出大小均匀,粒径约为7.8 nm的呈正电性的银纳米颗粒。在预先梳直的小牛胸腺DNA模板上合成了长约2μm、直径约30 nm、银颗粒较均匀分布的银纳米线。拉曼光谱表明:银颗粒主要结合在DNA主链上,同时脱氧核糖和碱基的振动峰也受到影响。磷酸根骨架的伸缩振动峰782和1098 cm-1的强度明显减少,且谱线782移动到791 cm-1。脱氧核糖C—O的伸缩特征峰1011和1050 cm-1分别移动到1030和1064 cm-1。碱基的特征峰1372,1334,1304和728 cm-1分别移动到1368,1320,1294和731 cm-1。

Silver nanoparticles were synthesized by the method of combining heating with UV irradiation. In the authors＇ work, the shape and size of nanograins which carry positive electrical charges are uniform and their average diameter is 7. 8 nm. Based on the electrostatic self-assembly characteristics of DNA, silver nanoparticles were equally assembled on predefined aligned calf thymus DNA to form DNA silver nanowires. The diameter of the wires is about 30 nm and the length is 2 pan. The Raman spectra indicate that the silver nanoparticles mainly attach to the backbone chain of DNA and affect the vibration properties of deoxyribose and base. The intensity of the peaks at 782 and 1 098 cm^-1 assigned to stretch vibration of phosphoric skeleton decreases sharply and the band at 782 cm^-1 shifts to 791 cm^-1. The bands of deoxyribose C-O stretch vibration at 1 011 and 1 050 cm^- 1 shift to 1 030 and 1 064 cm^-1 respectively. The characteristic peaks of bases at 1 372, 1 334, 1 304 and 728 cm^-1 shift to 1 368, 1 320, 1 294 and 731 cm^-1 , respectively.