转反义ACS基因番茄和普通番茄果实中锌含量和钠钾比与抗病性的关系研究

Relationship of Zn Content and Na/K Ratio to the Resistance to Pathogen Invasion in Antisense ACS Transgenic Tomato Fruit and Normal Tomato

植物激素与矿质元素吸收以及与植物抗性的关系研究是近年来人们关注的热点。实验采用电感耦合等离子体原子发射光谱法(ICP-AES)测定转反义ACS基因耐贮番茄(乙烯的生物合成被抑制)及同品种普通番茄果实(对照)中Zn,Na,K元素的含量及Na/K比例的差异,并对其与果实抗病原菌侵染能力的关系进行讨论。结果表明:乙烯生物合成受阻的转反义ACS番茄果实抗病原菌侵染的能力高于对照果实,其Zn元素的含量是普通番茄果实的1.5倍;K元素含量在两种果实中无明显差异,但Na元素含量显著高于对照,Na/K比值是对照的2.0倍。说明Zn元素含量和Na和K元素的比例关系可能在转反义ACS番茄果实抗病原菌侵染的过程中起到一定作用。

In the present work, inductively coupled plasma atomic emission spectrometry （ICP-AES） was used to determine the content of Zn, Na and K and the ratio of Na/K in antisense ACS transgenic tomato fruit （in which the antisense ACC synthase gene construct was inserted） and in normal tomato fruit of the same variety （Lycopersicon esculentum cv. Lichun）. The difference in mineral elements content between the two tomatoes and the involvement of Zn content and Na/K ratio in the disease resistance of postharvest tomato fruit were discussed. The results showed that the ethylene biosynthesis is limited in antisense ACS transgenic tomato fruit. Meanwhile, the resistance to Rhizopus nigricans invasion of this transgenic tomato was higher, the disease incidence and lesion area in transgenic tomato fruit were 20. 0% and 33.3% lower than those in control fruit, respectively. In addition, the content of Zn in transgenic tomato fruit was 0. 322 ug · g^-1 and was 1.5 times higher than that in Lichun tomato fruit. There was no significant difference in K content between transgenic tomato fruit and Lichun fruit, but the content of Na in transgenic tomato fruit was significant higher than that in Lichun fruit and the Na/K ratio in transgenic tomato fruit was 2. 0 times higher than that in Lichun fruit. It is suggested that the content of Zn and the Na/K ratio may be involved in the resistance response to pathogen invasion and the development of antisense ACS transgenic tomato fruit.