二氢丹参酮Ⅰ对 QGY-7703 细胞和 SPC-A-1 细胞放射敏感性的影响

THE INFLUENCE OF DIHYDROTANSHINONE Ⅰ ON THE RADIOSENSITIZATION EFFECT OF QGY 7703 AND SPC A 1 CELLS

利用体外细胞集落形成法研究二氢丹参酮Ⅰ对肿瘤细胞放射敏感性的影响。实验表明，二氢丹参酮Ⅰ对人肝癌细胞株（ＱＧＹ７７０３）和人肺腺癌细胞株（ＳＰＣＡ１）有较强的细胞毒作用，半致死剂量ＬＤ５０分别为８．８６μｍｏｌ／Ｌ和７．７３μｍｏｌ／Ｌ。该药物与Ｘ射线联合作用能增强细胞对射线的敏感性，１μｍｏｌ／Ｌ的二氢丹参酮Ⅰ作用于有氧细胞１ｈ后照射，对ＱＧＹ７７０３细胞和ＳＰＣＡ１细胞的增敏比（ＳＥＲ）分别为１．４２和１．６２；ＱＧＹ７７０３细胞和ＳＰＣＡ１细胞的氧增比（ＯＥＲ）分别为２．０８和３．０３。１μｍｏｌ／Ｌ的二氢丹参酮Ⅰ在乏氧状态下对这两种细胞亦有放射增敏作用，ＱＧＹ７７０３细胞的ＳＥＲ仍为１．４２，而ＳＰＣＡ１细胞的ＳＥＲ增大为１．９６。在分次照射实验中，１μｍｏｌ／Ｌ的二氢丹参酮Ⅰ能完全抑制这两种细胞的亚致死性损伤修复（ＳＬＤＲ）。

In the experiments, the radiosensitization of dihydrotanshinone Ⅰ on human hepatocelluar carcinoma cells QGY 7703 and lung adenocarcinoma cells SPC A 1 was studied through in vitro colony formation method. The results showed that dihytanshinone Ⅰ has cytotoxity to the two kinds of cells and the LD 50 was 7.73μmol/L and 8.86μmol/L, respectively. The compound enhanced the radiation effect. Under aerobic condition the SER (sensitizing ehnancement ratio) of the compound was 1.42 for QGY 7703 cells and 1.62 for SPC A 1 cells at the concentration of 1μmol/L. The OER (Oxygen enhancemen tratio) for QGY 7703 cells and SPC A 1 cells was 2.08 and 3.03, respectively. Under hypoxic condition dihydrotanshinone Ⅰ also had radiosensitivity for the two kinds of cells, and the SER of SPC A 1 cells increased to 1.96 while the SER of QGY 7703 kept 1.42. In split dose radiation experiment, dihydrotanshinone Ⅰ could inhibit SLDR (sublethal damage repair) obviously at the concentration of 1μmol/L.