2个不同类型尖叶胡枝子的微形态和分子标记鉴定

Micromorphology and Molecular Marker Identification of Two Different Types of Lespedeza hedysaroides

通过扫描电镜观察到叶片浓绿型和叶片黄绿型2个不同类型尖叶胡枝子的豆荚和种皮微形态具有明显差异,其特征可作为2个不同类型尖叶胡枝子的鉴定依据。还采用ISSR-PCR分子标记方法对2个不同类型尖叶胡枝子进行了鉴定。实验共考察8个引物,筛选出引物UBC-880可以独立区分开2个不同类型的尖叶胡枝子,叶片浓绿型尖叶胡枝子可产生1条500~600 bp大小的多态性谱带,表明ISSR-PCR方法可为鉴定2个不同类型尖叶胡枝子提供DNA分子水平上的依据。

Two different types of L. hedysaroides with yellow-green leaf or dark-green leaf showed obvious differences in micromorphological features of pod-coat and seed-coat by scanning electron microscope （SEM） observation. These micromorphological features can be used for identification of two different types of L. hedysaroides. ISSR-PCR molecular marker was also used to identify two different types of L. hedysaroides. Among eight primers for experiments, the primer UBC-880 was able to distinguish the examined two different types of L. hedysaroides. L. hedysaroides with dark-green leaf has a polymorphic band in 500 - 600 bp. ISSR-PCR molecular marker can be used to identify two different types of L. hedysaroides on DNA level.