一株嗜热纤维素分解菌的分离及其酶学性质的初探

Initial Studies on Isolation of a Thermophilic Cellulose-degrading Bacteria and its Enzymology Characteristics

利用海南热泉附近腐烂朽木及土壤,筛选分离到一株具有较高纤维素酶活性的纤维素分解菌WQ-50,经生理生化特性及遗传分析初步鉴定其为环状芽孢杆菌属菌株。当以复筛培养基发酵培养时,该菌株在55℃、pH 6条件下生长较好。在该条件下,以经碱预处理的玉米秸秆粉为唯一碳源发酵培养5 d时产酶达到最大值,羧甲基纤维素酶活性为6.8 IU/mL,滤纸酶活性为3.5 IU/mL。

WQ-50 was isolated from decayed wood and soil near hot spring in Hainan. It was identified as Bacilluscirculans by preliminary physiological and biochemical characteristics and genetic analysis. When fermented by CMC-Na culture medium, strain WQ-50 strain could grow well under PH 6 and 55℃. Under this condition, when fermented by zymolytic culture medium with alkalized pretreated corn stalk powder as the sole carbon source, enzyme production reached maximum after 5 days, the CMCase activity of WQ-50 is 6.8 IU/mL and FPA activiy of WQ-50 is 3.5 IU/mL.