青钱柳叶总黄酮超声辅助提取工艺优化

Technology Optimization of Total Flavonoids Extraction from Cyclocarya Paliurus Leaves by Ultrasonic Assistance

为研究以乙醇为溶剂超声波辅助提取青钱柳叶总黄酮的工艺,在单因素试验的基础上,采用四因素二次回归正交旋转组合设计,以液料比、超声处理时间、超声功率、溶剂体积分数为考察因素,优选了超声波辅助提取青钱柳叶总黄酮的最佳工艺。结果表明各因素对提取率的影响大小依次为:超声处理时间、溶剂体积分数、液料比、超声功率。最佳工艺条件为液料比55 mL/g,超声处理时间34 min,超声功率720 W,溶剂体积分数60%,在此最佳条件下,总黄酮的得率为86.63%,优化工艺稳定,提取率也较高,试验验证结果与模型预测值相符。

The supersonic assistance extraction processing of total flavonoids from Cyclocarya paliurus leaves was studied. By means of orthogonally rotational combination design with four factors （ratio of solvent to dry Cyclocarya paliurus leaves, extraction time, ultrasonic power and ethanol concentration） as influence factors, the optimal extraction technology was performed. The results indicated that the effect of factors on the extraction was in the order as follows, extraction time, the most important one, then ethanol concentration, ratio of solvent to dry Cyclocarya paliurus leaves, and ultrasonic power as the last one. The optimum conditions were ratio of solvent to dry Cyclocarya paliurus leaves 55 mL/g, extraction time 34 min, ultrasonic power 720 W, and ethanol concentration 60 percent. With the optimized technology, the content of total flavonoids in the final product is 86.63 percent. In conclusion, the technology is relatively stable and reliable, and consistent with the validate experiment result.