高效液相色谱分析不同低氧训练模式下大鼠心肌组织腺苷酸含量变化特征

Determination of Adenosine Phosphates Content in Rat Myocardium after Different Pattern of Hypoxic Training by High Performance Liquid Chromatography

目的:利用高效液相色谱(HPLC)技术,探讨不同低氧训练模式下大鼠心肌组织ATP、ADP、AMP含量以及能荷(EC)水平能量代谢的变化特征。方法:将40只大鼠随机分为4组,每组各10只,分别进行常氧训练(control)、高住低练(HiLo)、低住高练(LoHi)和间歇性低氧训练(IHT),4周后断头处死,取心肌组织进行液相色谱分析。结果:不同低氧训练模式下大鼠心肌组织ATP、ADP、AMP含量以及EC水平变化存在一定差异,与常氧训练相比,HiLo组ATP下降了36.1%,LoHi组和IHT组分别提高了12.5%和36.0%,均产生显著性差异;ADP和AMP的变化趋势与ATP相似,除了HiLo组AMP下降了23.9%外,其余的均未有较大的变化;HiLo组心肌组织腺苷酸池总量下降了19.0%,而LoHi组和IHT组表现为略有提高,但只有IHT组产生显著性差异(P<0.05),各组的EC水平差异不大,维持在相对稳定的状态。结论:HiLo低氧训练后的ATP含量、腺苷酸池总量以及EC水平最低,IHT低氧训练后的ATP含量、腺苷酸池总量以及EC水平最高,表现出IHT训练后EC维持能量代谢能力的效果最为显著,EC水平维持能量代谢能力表现为IHT>LoHi>常氧训练>HiLo。

Objective： The purpose of this study was to explore the changes of contents of ATP, ADP, AMP and EC in rat myocardium after different pattern of hypoxic training with high performance liquid chromatography （HPLC）. Method：Forty rats were randomly divided into four groups with 10 rats in each group, which made four weeks training for low altitude training （control）, living- high-training-low （HiLo）, living-low-training-high （LoHi） and interval hypoxic training （IHT）. Rats were killed by neck breaking and myocardium was determined by HPLC four weeks later. Result：There was existed certain different on changes of contents of ATP, ADP, AMP and EC in rat myocardiurn after different pattern of hypoxic training. Compared with control group, content of ATP in HiLo decreased 36. 1%, but LoHi and IHT group＇s increased 12. 5 % and 36.0 % respectively, there were significant differences among groups. Except the contents of AMP and adenosine phosphates pool in HiLo group decreased 23.9% and 19.0%, there were no differences among groups, although the energy charge of each group was not improvement degree vary from difference training model. Conclusion： The contents of ATP, adenosine phosphates pool and EC were the lowest in HiLo group but the highest in IHT group after four weeks hypoxic training. This result indicated that IHT was the best hypoxic training model to improve the level of energy metabolism, as to EC, IHT〉LoHi 〉 control〉 HiLo.