采油废水模拟处理系统中酵母菌群落结构动态初步解析

Analysis on Yeast Population Dynamics of Simulated Oilfield Produced Water Biotreatment System

本研究建立好氧活性污泥的模拟体系处理经过物化预处理的采油废水,控制温度等条件接近实际运行体系的情况下,考察系统COD去除率和污泥体系中酵母菌多样性变化。结果显示系统稳定后COD去除率在70%以上;利用非培养的方法考察了污泥中酵母菌在系统启动和稳定后的多样性变化,结果表明酵母菌在采油废水处理系统中有较高的多样性和稳定性;原水中具有较高的酵母菌多样性,并在系统稳定过程中呈现逐渐增加的趋势。表明酵母菌可以在活性污泥系统中稳定存在,同时酵母菌等真核微生物在烃类污染物去除和环境治理上具有一定的研究和应用价值。

The simulated activated sludge system was set up to treat the oilfield produced water after physi- cal-chemical treatment in this study. The molecular methods such as PCR-DGGE and sequencing were ap- plied to study population dynamics and diversity of yeast during system starting up and running. The result indicated that both in the influent and activated sludge showed higher yeast diversity. And yeast diversity in- creased during system running. It suggested that yeast would exist in the sludge and contribute to COD re- moval. And also yeast would perform well in hydrocarbon transformation and pollutant removal.