龙须菜几株附生细菌对铜离子吸附性能的比较

Comparison Copper Absorption Capacity with Several Bacteria Stains from the Surface of Macroalga Gracilaria lemaneaformis(Rhodophyta)

重金属污染和富营养化是沿岸海域的主要污染形式,造成沿海生态功能的退化。在重金属污染区域往往伴随着富营养化现象。本文对分离来自电镀废水中铜污染海域内的龙须菜体表的附生微生物对Cu2+的生物吸附进行试验,分析和比较这些微生物对Cu2+的生物吸收性能。共分离到6株优势附生细菌:人苍白杆菌GF-S1、杀鲑气单胞菌GF-S2、莱拉微球菌GF-S3、纹带棒杆菌GF-S4、假交替单胞菌GF-S5和弗氏弧菌GF-S6。经对Cu2+的生物吸附试验发现,GF-S2效果最好,GF-S6最差,其余间于这两者之间。对GF-S2、GF-S4和GF-S6进行了最佳吸附时间和pH筛选,以及使用不同化学试剂对细菌Cu2+吸附的影响试验。结果显示:最佳吸附时间在40min～60min,pH介于4～5对GF-S2、GF-S4吸附Cu2+的效果较好;HCl处理导致GF-S2丧失吸附能力,NaOH和乙醇能提高其吸附性能;3种试剂都能较大地提高GF-S4和GF-S6的吸附性能,尤其对GF-S4经处理后,对环境中的Cu2+的去除率得以大幅度提高,去除较彻底。

The main pollution of coastal sea is heavy metal and eutrophication, which resulted in degeneration of its eco-function. Pollution of heavy metal is accompanied with eutrophication in sea water. This arti- cle studied on copper absorption capacity of several bacteria strains which were isolated from the surface of macroalga Gracilaria lemaneaformis that was cultivated in copper polluted sea. We got 6 strains of epiphytic bacteria from the seaweed, Ochrobactrum anthropi, Aeromonas salmonicida, Micrococcus lylae, Coryne- bacterium ulcerans, Pseudoalteromonas spp, Vibrio fluvialis. Attained from the experiment of copper bio- sorption, A. salmonicida had a maximal biosorption capacity among them, and Vibrio minimal. Based on this trail, A.salmonicida, C. ulcerans and Vibrio were selected for next trails included optimal biosorption time, optimal pH value and effect of pre-disposal by several chemical reagents on biosorption capacity, The results showed： optimal biosorption time was between 40 minutes and 60 minutes, and ootical nH value 4-5.Pre-disposal by HCI leaded to lost the absorption capacity of A. salmonicida, but was opposite by NaOH and ethanol. For C. ulcerans and Vibrio, the copper absorption capacities were increased by them.