基质金属蛋白酶-3在原发性干燥综合征患者唇腺中的表达

Expression of MMP-3 in labial glands of primary sjogren′s syndrome patients

目的:研究基质金属蛋白酶-3(matrix metalloproteinases-3,MMP-3)在原发性干燥综合征(primarySjogren′s syndrome,pSS)患者唇腺组织中的表达情况。方法:收集24例pSS患者和14例对照组的唇腺标本,提取唇腺中总RNA,利用反转录聚合酶链反应(RT-PCR)法扩增MMP-3、β-肌动蛋白(β-actin)的片段,计算MMP-3/β-actin吸光度比值。免疫组织化学染色方法检测MMP-3蛋白在唇腺中的表达情况。结果:pSS组唇腺中MMP-3的mRNA转录及蛋白表达水平均高于对照组(P<0.05)。MMP-3蛋白主要分布在腺体导管上皮细胞胞浆内。结论:pSS患者唇腺中MMP-3表达增加,可能是唇腺结构破坏的原因之一。

Objective： To investigate the mRNA transcription and protein expression of MMP-3. Possible roles of MMP-3 in primary sjogren＇s syndrome（pSS） were analyzed. Methods： For this study, 24 patients with pSS were selected and diagnosed by the American-European Consensus Group standards. The control group was composed of 14 subjects. Total RNA from labial glands was extracted. The target genes and β-actin acted as templates to perform reverse transcript polymerase chain reaction （RT-PCR）. Then mRNA expression of target genes was semi-quantitated by the OD ratio of the target genes to β-actin bands on gel under densitometry. Immunohistochemical analysis was made to determine the expression of proteins of targets genes in labial glands of pSS patients and control group. Results： In contrast to control group, the mRNA transcription and protein level of target genes in labial glands of pSS patients were significantly increased statistically （ P 〈 0.05 ）, and MMP-3 protein was mainly expressed in intracytoplasm of gland duct. Conclusion： The increase of MMP-3 in pSS patients provides a rationale for understanding the organization observed in the basal lamina and apical surface of acini in these patients.