内毒素致兔急性肺损伤及血必净保护作用

Endotoxin-induced acute lung injury in rabbits and protective effect of Xuebijing

目的:观察内毒素(ET)致兔急性肺损伤(ALI)时肺组织中丙二醛(MDA)含量及超氧化物岐化酶(SOD)活性的的变化,探讨血必净对急性肺损伤保护作用的机制。方法:将24只日本大耳白兔随机分为对照组(A组),内毒素致伤组(B组),内毒素致伤及血必净干预组(C组)。用内毒素(750μg/kg)一次性静脉注射方法复制兔ALI模型,应用血必净按1.8mL/kg静脉注射进行干预,分别检测肺组织中的MDA含量及SOD活性。结果:A组氧合指数保持稳定,B组在致伤后0.5h动物氧合指数显著下降,之后略有回升但仍低于基础水平,C组下将趋势低于B组。B组动物肺组织中MDA含量明显高于A组(P<0.01)与C组(P<0.05),A组、C组间无差别。B组肺组织SOD活性明显低于A组(P<0.01)与C组(P<0.05),A组、C组间无差别。肺组织病理学发现A组肺组织正常,B组出现渗出,淤血,炎细胞浸润等,C组病理损伤轻于B组。结论:血必净可以降低肺组织中MDA的含量和提高SOD的活性,该药对内毒素所致兔ALI有一定的保护作用。

Objective： To investigate the changes of maleic dialdehyde （MDA） levels and superoxide dismutase （SOD） activities in the lung tissues of rabbits with eudotoxiu-induced acute lung injury （ALI） , and study the protective mechanism of xuebijing. Methods： 24 male Japanese flap-eared white rabbits were randomly divided into 3 groups： control group （Group A） , endotoxin-indueed ALl group （Group B） and xuebijing treatment group （Group C ）. ALl models of the rabbits were replicated by intravascular ET injection （750p, g/kg）, and xuebijing （1.8mL/kg weight）was intravascularly injected in xuebijing treatment group. Results： MDA levels in-Group B was significantly higher than in Group A（P 〈0.01） and Group C （P 〈0.05） , and there was no difference between Group A and Group C. SOD activities in Group B were lower than in Group A （ P 〈 0.01 ） and Group C （ P 〈 0.05 ） , and no difference was found between Group A and Group C. Conclusion： Xuebijing can significantly lower MDA levels and increase SOD activities in lung tissues. Xuebijing may have the effect of protecting rabbits with endotoxin-induced ALL