人胚胎干细胞在3种培养体系中的生长

Growth of Human Embryonic Stem Cells in Three Culture Systems

人胚胎干细胞(human embryonic stem cells,hESCs)的培养一直是干细胞研究的重要内容.用本实验室独立建系的两株hESCs,建立3种不同的培养体系:小鼠胚胎成纤维细胞(mouse embryonic fibroblasts,MEFs)做饲养层,永生化人成纤维细胞(immortalized human adult fibroblasts,HAFi)做饲养层,无饲养层条件培养基培养体系(condition medium,CM),观察在3种培养体系中,干细胞的增殖和分化情况.发现3种培养体系中的hESCs都可以表达一致的生物学特性,但也有不同之处,相对于CM干细胞在MEFs和HAFi饲养层体系的分化率低,增殖快;但MEFs来源于鼠类是异源细胞,HAFi虽不含鼠源性成分却繁殖很慢;无饲养层的体系便于操作,无外源细胞存在.实验所得出的结果可以引导研究人员针对于临床、科研不同的需要,选择最适合的培养体系.

Culture systems for human embryonic stem cells （hESCs） were important for hESCs researches. Three different culture systems were used as follows： mouse embryonic fibroblasts （MEFs） as feeder-layer, immortalized human adult fibroblasts （HAFi） as feeder-layer and feeder-free system with condition medium （CM）. The two cell lines were established in Our laboratory. Information of growth and differentiation of hESCs were detected. It was found that both lines could maintain all their characteristics in these three culture systems. But there were also differences： differentiated rates were lower and rates of propagation were higher in MEFs and HAFi than CM; MEFs obtained from mice were heterogeneous cells; HAFi was human resources but grew too slowly; feeder-free system reduced the labor and was pure in relative. The results of the study provided useful data for a proper culture system in clinical and reseach Works.