卡介苗菌基因组DNA对小鼠腹腔巨噬细胞激活效应的初步研究

Primary Study on the Action of Genome DNA of Mycobacterium Bovis-Bacille Calmette-Guerin for Macrophage Activation in Mice

为了比较研究卡介苗菌基因组DNA和卡介苗菌激活小鼠腹腔巨噬细胞的抗结核免疫应答过程及免疫作用,用卡介苗菌基因组DNA、卡介苗菌和生理盐水分别皮内注射小鼠第30、60d后,分别采用Griess法、化学法、ELISA法检测小鼠腹腔巨噬细胞产生的NO、H2O2以及IL-12、TNF-α的表达.研究结果,卡介苗菌基因组DNA和卡介苗菌皮内接种小鼠后能显著诱导小鼠腹腔巨噬细胞分泌表达IL-12和TNF-α,卡介苗菌基因组DNA组和卡介苗菌组相比较,无明显差异,与生理盐水组比较,其差异均有统计学意义(P<0.05);卡介苗菌基因组DNA和卡介苗菌皮内接种小鼠后也能诱导小鼠腹腔巨噬细胞产生NO、H2O2,卡介苗菌基因组DNA组和卡介苗菌组相比较,无明显差异,与未免疫组比较均具有统计学意义(P<0.05).研究结果表明,卡介苗菌基因组DNA皮内接种小鼠后能诱导小鼠腹腔巨噬细胞活化并产生较强的激活效应,且该效应于卡介苗菌无明显差异.

In order to study on immunity responses of the geneome DNA of bovis-bacille calmette-guerin （BCG） and BCG, and the function of anti-bacterium immunity of macrophages, male C57BL/6 mice were immunized intracutaneously with the geneome DNA of BCG and BCG. The production of nitric oxide（NO） and H202 as well as the expression levels of IL-12 and TNF-α of the IFN-y-stimulated or un-stimulated mouse celiac macrophages were determined by Griess＇s method, chemical method and ELISA assay respectively. 30 days and 60 days after the intracutaneous vaccination, it was found that immunization of the geneome DNA of BCG could induce macrophages effectively to secrete IL-12 and TNF-α with significant difference to the un-immunized group, but without any difference with BCG -immunized group of mice. In addition, it also induce macrophages to produce NO and H202 with significant difference to the un- immunized group, but without any difference with BCG -immunized group of mice. IFN-T demonstrated an intensive effect on the production of NO and cytokines from macrophages. The results show： it is concluded that the intracutaneous vaccination with the geneome DNA of BCG can induce activation of macrophages and generate strong immune responses, and this effect is not significantly different from that of immunization with BCG.