山西汉族Y-STR基因座DYS552遗传多态性调查

Polymorphism of DYS510 in a Chinese Han population of Shanxi

目的：调查Y染色体上的短串联重复序列DYS552基因座在山西汉族男性人群中的基因频率分布、多态性及群体分布情况，为法医学个体识别及亲子鉴定提供计算数据，对其在法医学上的应用进行可行性探讨。方法：随机抽取102例山西汉族男性无关个体静脉血500uL，用GDB中的引物进行PCR扩增，银染显色，对其选取的等位基因测序，构建等位基因分型标准物；按照ISFG原则命名各等位基因。结果：102例山西汉族人群DYS552基因座共检出7种不同的等位基因，分别为DYS552×23（0．0194）、DYS552×24（0．1105）、DYS552×25（0．3981）、DYS552×26（0．2427）、DYS552×27（0．1262）、DYS552×28（0．0874）、DYS552×29（0．0097），基因多样性为0．7523，其个人识别能力（DP）和非父排出率（PE）均为0．7523。结论：DYS552基因座具有较高的遗传多态性，在法医学及人类遗传学方面具有较高应用价值。

Objective ：To provide the evidence for the application of DYS552 in forensic science and population genetics by investigating its polymorphism in the Han populmion lived in Shanxi province. Methods ：To collect respectively 102 unrelated male＇s venous blood 500 uL,who live in Shanxi province. The products of PCR were analyzed with polyacrylamide gel electrophoresis. Each allele of this Y-STR loci was sequenced and the allele ladders were constructed. Alleles of this Y-STR loci were nominated according to recommendations of the International Society of Forelnsic Genetics. Results：There are total 7 alleles in DYS552 site in Shanxi Han population,called DYS552× 23, DYS552 ×24.DYS552 ×25.DYS552 × 26.DYS552 × 27,DYS552 × 28,DYS552 × 29 ,the GD value is 0. 752 3. If used in forensic science,both of the power of diacrimination（PD）and the power of exclusion（PE）were 0. 7523. Conclusion： DYS552 has a high level of genetic polymorphism and can be used in forensic science and human genetics research.