3种方法联合运用提取人体脱落上皮细胞DNA

The Application of Three Methods Combined for Extracting DNA from Human Epithelium

目的寻求提高法医物证检验中附有人体脱落细胞载体检材的DNA检出率的方法。方法根据案件检材的具体条件,选择3种提取方法即Chelex-100法、Chelex-100联合有机法和Chelex-100联合磁珠法。结果采用了分步提取DNA法,可充分、有效地提取微量DNA,提高了人体脱落上皮细胞有效DNA的检出率。结论根据第一步提取方法的检测结果,来调整下一步的提取策略,如是否加以纯化浓缩,可增加人体脱落上皮细胞有效DNA的检出率。

Objective To improve DNA extraction from human epithelium. Methods Chelex-100, Chelex- 100 organic combined and Chelex-100 magnetism pearl combined methods were adopted according to the situation of each case. Results With the stepwise extracting protocol, the trace amount of DNA was analyzed most efficiently and the success rate of DNA extracting was better improved. Conclusion The efficiency of DNA extraction from human epithelium could be greatly improved by modifying the extracting protocol such as purification and concentration based on previous results.