葛根提取物对酒精性肝损伤大鼠肝组织TNF-α表达变化的研究

On the Expressive Changes of the Rat's Alcoholic Liver Injury 's TNF-a Caused by the Extractive from the Root of Kudzu Vine

目的:观察葛根对酒精性肝损伤大鼠肝组织肿瘤坏死因子-α(Tumor Necrosis Factor-α,TNF-α)水平的变化。方法:以56%酒精灌胃,造成大鼠酒精性肝损伤模型,取肝组织做免疫组织化学检测TNF-α表达。结果:模型组和治疗对照组大鼠毛发光泽差,食欲差,大便溏泻,肝组织出现肝脂肪变性,肝组织中见大量TNF-α蛋白阳性产物棕褐色细颗粒;治疗组肝组织中见少量脂滴空泡,少量TNF-α蛋白阳性产物棕褐色细颗粒。结论:葛根提取物能降低酒精性肝损伤大鼠肝组织TNF-α的表达。

Objective： To observe the level changes of the rat＇s alcoholic injury＇s TNF-α caused by the extractive from the root of kudzu vine. Methods： Filling the rat＇s stomach with alcohol （56%） to form a model of alcohol liver injury so as to take liver tissue for immunohistochemistical test on the TNF-α expression. Results： Compared with the rats in the treated group, the rats in the model group are less appetitive and have loose stool, and their hair is less bright; fatty degeneration appear in their liver tissue and in it also appear a lot of brown particles of TNF-α protein active products; while in the treated group, there are fewer such panicles and lipid droplet vacuoles. Conclusion： The extractive from the root of kudzu vine can reduce the expression of rat＇s alcoholic liver injury＇s TNF-α.