摘要
采用酶细胞化学技术对7个甘蔗种和品种进行研究。甘蔗茎韧皮部细胞 ATP 酶活性定位于筛管、伴胞质膜、伴胞核、小囊泡、充分发育的液泡膜和 P—蛋白上。野生种和栽培种茎韧皮部细胞 ATP 酶活性较高,而生产品种则较低。认为甘蔗茎韧皮部 ATP 酶活性与糖分的运输和抗性等有关。茎韧皮运输中,可能有 P—蛋白和 ATP 酶主动参与。
A comparative study on the ultrastructural localization of ATPase activity in the cells of the fifth internode of different species was carried out with cytochemical technique.In the phloem cells of sugarcane stem,ATPase activity was localized on the plasma membrane of sieve elem- ents and companion cells,the nuclei,vescles,full development vacuole of companion cell and P-protein.The ATPase activity showed obvious diffe- rence in the stem phloem cells of varous species.The ATPase activity in the phloem ceils of wild species and original cultigens was higher than that of commercial varietes.The results suggested that the phloem ATPase acti- vity in the sugarcane stem be associaed with the transport and accumulation of sugar and the resistibility,and there be P-protein ATPase taking part in the phloem transport of sugarcane stem.
关键词
甘蔗
ATP酶活性
细胞化学定位
sugarcane
ATPasc activity
ultastructural localization
