蒙脱石散稳定肥大细胞功效治疗腹泻型IBS

Montmorillonite Powder's effects on DP-IBS by stabilizing mast cells

目的探讨蒙脱石散吸附细菌潜生体(CGC)、排除CGC定植、稳定肥大细胞治疗腹泻型肠易激综合征(IBS)的效果。方法蒙脱石散体外吸附CGC、防止肥大细胞脱颗粒,体内排除情况,拮抗肥大细胞增多和脱颗粒试验观察其效果。结果蒙脱石散体外与细菌CGC作用后,细菌CGC被吸附在蒙脱石散颗粒周围;高、中、低剂量的蒙脱石散体外防止肥大细胞脱颗粒,其肥大细胞脱颗粒率〔(18±0.88)%,(21±0.93)%,(24±2.12)%〕和模型组(32±2.83)%比较差异有统计学意义(P<0.01);高、中、低剂量的蒙脱石散治疗IBS,给药后4d,中、高剂量组CGC开始减少,第7天低剂量组见少量CGC定植,中、高剂量组未检出CGC;治疗7d结束后高、中、低剂量组治疗组肥大细胞脱颗粒率〔(45.3±5.2)%,(48.0±4.8)%,(61.7±6.0)%〕和模型组(78.8±4.6)%比较差异有统计学意义(P<0.01)。结论蒙脱石散能减少CGC定植,拮抗肥大细胞脱颗粒。

Objective To investigate the effects of Montmorillonite Powder on DP-IBS. Methods Both the in vitro and in vivo experiments were conducted to explore the effects of Montmorillonite Powder on the bacterial CGC distribution,mast cells degranulation,loeal colonization of bacterial CGCs in colon of IBS model rats at different dose. Results Montmorillonite Powder exhibited the role of absorption bacterial CGC and prevention of mast cell degranulation in vitro, the degranulation rate of high, mediate and low dose groups were （18±0.88）%, （21±0.93）%, （24±2.12）% respectively,and showed significant difference to model groups （32 ±2.83）%, （P 〈0.01） ;on 4d after the treatment with Montmorillonite Powder,the high and mediate dose groups showed the decrease of bacterial CGC in fecal, after 7d, there was a few CGCs found to be colonization on the mucosa in low dose group,whereas no colonization of CGCs was found in rats of mediate and high dose groups. In the meantime, the mast cell degranulation rates were also showed significance difference with that of IBS model rats （P〈0.01）. Conclusion Montmorillonite Powder can reduce the bacterial CGC distribution and mast cells degranulation.