高效液相色谱法测定人血浆中兰索拉唑浓度

Determination of lansoprazole in human plasma by HPLC

目的建立测定人血浆中兰索拉唑浓度的高效液相色谱方法。方法血浆样品经二氯甲烷和乙醚的混合提取液提取后,以乙腈:0.05mol/L磷酸二氢氨溶液(pH7.0,35:65)为流动相,经PAPCELLPAK-C18色谱柱分离,于285nm波长处检测。结果兰索拉唑浓度在0.025～2.0μg/mL范围内线性关系良好,最低检测浓度为0.025μg/mL;低、中、高3个浓度的提取回收率均大于74%,日内、日间相对标准差低于8.22%。结论本方法准确、快速,可满足临床药代动力学研究的要求。

Objective An I-IPLC method was established for determination of lansoprazole in human serum. Methods Serum protein was precipitated with trichloroacetic acid. The analysis was conducted with a Diarnonsil-C18 （4. 6 mm× 150 mm,5 um）column, acetonitrile -0. 05 moVL citric buffer（pH4.0,18：82） was served as mobile phase, the detecting wavelengh was at 310 am. Results The linear range lansoprazole was 0. 025 - 2. 0 ug/mL （ n = 3, r = 0. 997 7）and the average recovery was 74. 0% -81.5% at three different concentrations. The RSD of intra- and interday were less than 8. 22%. Conclusion This method is suitable for the phafrnacokinetic study of lansoprazole.