山羊十二指肠内灌注酸或碱对血浆胃动素水平的影响

Effect of Intraduodenal Infusion of Acid or Alkali on Goat Plasma Motilin

选用２只装有永久性十二指肠瘘管的１周岁母山羊，禁食２４ｈ分别以５ｍＬ·ｍｉｎ－１的速度经瘘管灌注０１ＭＨＣ１（ｐＨ为１０），０３ＭＴｒｉｓ（三羟甲基氨基甲烷）缓冲液（ｐＨ为１０５）和生理盐水（ｐＨ为３９）各１００ｍＬ，重复进行２次，每隔７２ｈ灌注１次。分别于灌注后０，５，１０，２０，３０，６０，９０ｍｉｎ采集颈静脉血样，用放射免疫法测定血浆胃动素水平。结果如下：（１）山羊禁食２４ｈ无灌注时血浆胃动素水平（即基础水平）为（１６８４４～２３４１７）ｎｇ·Ｌ－１，平均为（２０１０４±２５３１）ｎｇ·Ｌ－１（２）灌注０１ＭＨＣ１时，血浆胃动素水平由基础水平显著降至（１６８９５±２１８５）ｎｇ·Ｌ－１（Ｐ＜００５），并稍低于灌注生理盐水（１７３０４±１４３５）ｎｇ·Ｌ－１（Ｐ＞００５）。（３）灌注０３ＭＴｒｉｓ缓冲液时，血浆胃动素水平由基础水平显著升高至（２５４２２±５４４１）ｎｇ·Ｌ－１（Ｐ＜００１），且明显高于灌注生理盐水（Ｐ＜００１）。表明酸化山羊十二指肠内环境能明显降低血浆胃动素水平，碱化则起显著升高作用。

Two one yeal old female goats were installed with permanent duodenal fistulas.After fasting for 24 hours,100mL 0.1M hydrochloric acid,100mL 0.3M Tris buffer and 100mL saline solution were separately infused into duodenal at 5mL·min -1 by fistulas,repeating the above procedure twice and once for every 72 hours.The blood samples were synchronizedly taken from the goats′ neck vein cather at different time.Before and during the duodenal infusion,plasma motilin was measured by radioimmunoassay.The results of research show that:(1)The goat mean plasma motilin concentration (i.e.basic level) was (201.04±25.31)ng·L -1 after fasting for 24 hours but without infusion,the range was (168.44±234.17)ng·L -1 .(2)Infusing 0.1M hyd rochloric acid significantly lowered the plasma motilin to (168.95 ±21.85)ng·L -1 ,which was some what lower than that (173.04±14.35)ng·L -1 of infusing saline solution ( P >0.05).(3)When infusing 0.3M Tris buffer into the duodenal,the plasma motilin (254.22±54.41)ng·L -1 obviously increased from the basic level ( P <0.01),which was significantly higher than that of infusing saline solution( P <0 01) The results suggest that acidifying intraduodenal environmental of goats can significantly lower the plasma motilin,and the alkalization can obviously make it increase.