摘要
建立了一种高效液相色谱荧光检测法同时测定水产品中4种四环素族抗生素残留的分析方法。水产品样品用柠檬酸缓冲液提取,清液用Oasis HLB固相萃取柱净化,1.36%的KH2PO4溶液定容后,荧光检测器于λex=380nm、λem=520nm测定。土霉素、四环素在0.05~5.0mg/L范围内,金霉素、强力霉素在0.1~5.0mg/L范围内呈良好的线性关系,检出限分别为0.01、0.01、0.03、0.03mg/kg(S/N≥3),添加平均回收率80%~110%,相对标准差在2.0%-6.6%之间。具有方法简便、分离度高、检出限低的特点,满足常规检测及水产品安全控制的需要。
A high performance liquid chromatographic method was developed for simultaneous determination of four Tetracychne antibiotics (TCs) residues in aquatic products. Samples were extracted with C6 H8 O7 · H2 O buffer solutions and centrifuged. The sample extracts were cleaned up by Oasis HLB solid - phase extraction cartridge and then were determined by KH2PO4( 1.36% ) solution- FLD detection at λex = 380 nm, λem = 520 nm. The calibration graphs were linear in the concentration range of 0.05 - 2.5 mg/L, and 0.1 - 5.0 mg/L for OTC, TC and CTC, DOTC, respectively. The limits of detection were 0.01 mg/kg for OTC, TC, and 0.03 mg/kg for DOTC, CTC (S/N≥3). The average recovery is not less than 80.0%, with RSD in the range of 2.62% - 6.11%. The method is simple and reliable. It can be used to analyze the OTC, TC, CTC and DOTC residues in aquatic products with satisfactory results.
出处
《计量学报》
CSCD
北大核心
2008年第B09期238-241,共4页
Acta Metrologica Sinica
基金
浙江省重大科技计划项目(2007C13067)
关键词
计量学
高效液相色谱荧光检测法
四环素族抗生素
药物残留
水产品
Metrology
High performance liquid chromatography (HPLC)
Tetracychne antibiotics (TCs)
Residue of antibiotics
Aquatic product