比较初发和复发膀胱癌中bcl-2,bim表达的临床研究

Clinic research of comparing the expression of bcl-2,bim in maiden and recurrence papillary urothelial cell carcinoma of bladder

目的探讨bcl-2,bim表达在膀胱乳头状尿路上皮癌中的临床意义及其关系。方法采用RT-PCR法检测67例膀胱乳头状尿路上皮癌及20例正常膀胱组织中bcl-2,bim的表达情况。结果初发和复发膀胱乳头状尿路上皮癌组织bcl-2和bim的表达均高于正常膀胱组织(P<0.05或P<0.01)。与初发癌组织中表达水平相比,复发膀胱乳头状尿路上皮癌组织中bcl-2表达水平高(P<0.05),bim表达低(P<0.01)。Bcl-2和bim在膀胱乳头状尿路上皮癌组织中表达分别为(27.7±2.3)%和(90.0±6.8)%,呈负相关(r=-0.437,P<0.01)。膀胱乳头状尿路上皮癌细胞分化程度越低,临床分期越高,bcl-2表达越高(P<0.05),bim表达越低(P<0.05)。结论Bcl-2过表达、bim低表达在膀胱乳头状尿路上皮癌诊断及复发中可能有一定意义。

Objective To study the relationship and clinic appliance of the expression of bcl-2 and bim in papillary urothelial cell carcinoma of bladder. Methods Reverse transcriptase polymerase chain reaction （RT-PCR） was performed to evaluate the positivity of bcl-2 and bim in 67 cases of papillary urothelial cell carcinoma of bladder and 20 cases of normal bladder mucosa. Results Compared to maiden carcinoma cell, the expression of bcl-2 was higher （ P 〈 0.05 ） but bim was lower （ P 〈 0.01 ） in recurrence bladder cancer cell. There was a very low expression of bcl-2 and bim in normal bladder mucosa. The positive ratio of bcl-2 and bim was （27.7 ± 2.3 ）% and （90.0 ± 6.8 ）% in papillary urothelial cell carcinoma of bladder, they had negative correlation （r = -0. 437 ,P 〈 0.01 ）. The lower differentiation of papillary urothelial cell carcinoma of bladder and the higher grade of the clinic stages, the positive ratio of bcl-2 expression was higher（ P 〈 0.05 ）, but bim was lower（P 〈 0.05 ）. Conclusion The overexpression of bcl-2 and lower expression of bim have relations to diagnosis and recurrence of bladder.