单侧咀嚼大鼠三叉神经节内CGRPmRNA及其相应蛋白的表达研究

The expressions of CGRPmRNA and their proteins in trigeminal ganglia in rats with unilateral chew

目的观察磨除单侧后牙造成单侧咀嚼的大鼠三叉神经节内降钙素基因相关肽(CGRP)及降钙素基因相关肽信使核糖核酸(CGRPmRNA)的表达情况,进一步探讨颞颌关节病的发病机制。方法Wistar雄性大鼠48只,随机分为6组,包括3个实验组及相应的对照组,每组8只。实验组动物磨除右侧上、下颌磨牙.人为造成单侧咀嚼。双侧三叉神经节(TG)切片行CGRP免疫组织化学(SABC法)和原位杂交反应。光镜观察拍片,并用图像分析软件分别进行测定。结果与对照组对照,用SPSS10.0软件行统计分析。结果每一实验组咀嚼侧和非咀嚼侧TG内CGRP免疫阳性神经元百分比与各自对照组比较显著降低(P<0.01,P<0.05),其非咀嚼侧明显低于咀嚼侧(P<0.01,P<0.05)。原位杂交结果显示,每一实验组咀嚼侧与非咀嚼侧TG中CGRPmRNA阳性神经元的数量较各自对照组明显增高(P<0.01,P<0.05),其非咀嚼侧明显高于咀嚼侧(P<0.01)。结论三叉神经节内CGRP和CGRPmRNA参与了单侧咀嚼引起的颞颌关节病的病理过程,且两侧TG内CGRP释放量、CGRPmRNA合成量不同。

Objective To study the expressions of calcitonin gene-related peptide （CGRP） and CGRPmRNA in trigeminal ganglia in rats with unilateral chewing,and to discuss the mechanism of temporomandibular disorders. Methods Forty eight Wistar male rats were randomly divided into 6 groups,including 3 experimental groups and 3 control groups, 8 rats in each group . The molar of right maxillary and mandibular of rats in experimental groups were ground to the gingival level without occlusal contact. The section of trigeminal ganglia underwent the immunohistological and situ hybridization study. Light microscope and microscoic photo analytic software were employed to detect the percentage of CGRP and CGRPmRNA positive neurons in frozen section of TGs in 48 rats. SPSS10. 0 statistical software were used for statistical analysis. Results The percentage of CGRP positive neurons in TG in 3 experimental groups were significantly decreased compared with control group （P〈0.01 ,P〈0.05） ,The decreased extent in non-chewing side was much higher than that in the chewing side（P〈0.01 ,P〈0.05）. The percentage of CGRPmRNA positive neurons was higher in both chewing and nonchewing side of the experimental group than that in controls （P〈 0.01, P〈 0.05） ,and non-chewing side was much higher than that in the chewing side（P〈0.01）. Conclusion The CGRP and CGRPmRNA might participate in the histopathologic mechanism of temporomandibular disorders. The releaed amount of CGRP and the synthesis amount of CGRPmRNA in TG was different between chewing side and nonchewing under the condition of unilateral mastication.