5-氨基乙酰丙酸介导的光动力对CNE2和CNE2/DDP细胞体外作用的观察

Effect of 5-aminolevulinic acid mediated photodynamic therapy on CNE2 and CNE2/DDP in vitro

目的:观察5-氨基乙酰丙酸(5-ALA)介导的光动力对CNE2和CNE2/DDP的生物作用。方法:荧光分光光度计检测PpⅨ荧光强度;MTT法测定细胞存活率。结果:两种细胞内PpⅨ荧光强度与孵育时间(CNE2:F=65.56,P=0.000;CNE2/DDP:F=28.07,P=0.000)和5-ALA浓度(CNE2:F=6292.36,P=0.000;CNE2/DDP:F=914.36,P=0.000)成正相关,在相同孵育时间(t=17.28,P=0.01)及5-ALA浓度(t=36.07,P=0.000)条件下,CNE2胞内PpⅨ荧光强度均显著高于CNE2/DDP。CNE2或CNE2/DDP细胞存活率与孵育时间(CNE2:F=2245.98,P=0.000;CNE2/DDP:F=236.92,P=0.000)、5-ALA浓度(CNE2:F=55.26,P=0.000;CNE2/DDP:F=10.68,P=0.000)和激光能量(CNE2:F=3294.21,P=0.000;CNE2/DDP:F=285.65,P=0.000)成负相关,相同孵育时间(t=31.35,P=0.000)、5-ALA浓度(t=37.16,P=0.001)及激光能量(t=12.84,P=0.001)条件下,CNE2细胞存活率显著高于CNE2/DDP。结论:5-ALA-PDT对CNE2和CNE2/DDP细胞均具有杀伤作用,但在相同条件下,5-ALA-PDT对CNE2的杀伤作用大于CNE2/DDP。

OBJECTIVE： To investigate the potential use of 5-aminolevulinic acid for photodynamic therapy on CNE2 and CNE2/DDP. METHODS： The cellular fluorescence intensities of PpⅨ were measured with a luminescence spectrometer. The cellular survival rates were investigated with MTT. RESULTS： The cellular fluorescence intensities of PpⅨ increased with the 5-ALA incubation time （ CNE2： F = 65.56, P 0. 000 CNE2/DDP： F= 28.07, P = 0. 000） and 5-ALA concentration （CNE2：F=6 292.36, P 0.000; CNE2/DDP： F=914.36, P 0.000）. Under the same incubation time（t= 17.28; P= 0.01）or 5-ALA concentration（t=36.07, P--0. 000）, the cellular fluorescence intensities of Pp IX were lower in CNE2/DDP than those in CNE2 cells. Unlike sole incubation with 5-ALA or sole laser irradiation, the combination of both factors led to a significant time- （CNE2 ： F= 2 245.98, P = 0. 000 ; CNE2/DDP ： F=236.92,P=0. 000）,concentration-（CNE2： F=55.26, P= 0. 000; CNE2/DDP： F = 10.68, P = 0. 000） and energy-de- pendent（CNE2： F= 3 294.21,P= 0.000;CNE2/DDP：F= 285.65,P= 0. 000） decrease of cell survival rates. Under the same incubationtime（t=31.35,P 0.000）,5-ALA concentration（t= 37.16,P=0.001） or laser dosage（t= 12.84, P=0. 001 ）, the cell survival rates of CNE2/DDP were lower than those in CNE2 cells. CONCLUSION： Though 5-ALA-PDT may effective- ly kill CNE2 and CNE2/DDP cells cultured in vitro, CNE2/DDP cells are less sensitive to 5-ALA-PDT than CNE2 cells. Chin J Cancer Prey Treat, 2008,15 （22） ： 1702-- 1705