沙利度胺对人鼻咽癌细胞株血管内皮生长因子表达影响的观察

Expressions of VEGF and VEGF-C by thalidomide in human nasopharyngeal carcinoma CNE-2 cells

目的:探讨沙利度胺对人鼻咽癌细胞株体外生长及对血管内皮生长因子表达的影响。方法:采用MTT法检测沙利度胺对人鼻咽癌细胞增殖的抑制率;RT-PCR和免疫细胞化学方法分别检测沙利度胺作用前后血管内皮生长因子基因和蛋白表达水平的变化。结果:沙利度胺能抑制CNE-2细胞增殖,呈浓度依赖性,当60mg/L沙利度胺作用72h,其抑制率为45.6%;不同浓度沙利度胺作用后VEGFmRNA和VEGF-CmRNA表达均降低,VEGFmRNA下降最明显,P<0.05;蛋白表达与其相应mRNA呈正相关,r值分别为0.959和0.974,P值分别为0.035和0.021。结论:沙利度胺能够抑制CNE-2细胞增殖,其作用机制可能与其下调血管内皮生长因子的表达有关。

OBJECTIVE： To evaluate the effects of thalid- omide on the growth and expressions of vascular endothelial growth factors in human nasopharyngeal carcinoma CNE-2 cells. METHODS： The cell growth inhibition was assessed by MTT-mieroculture tetrazolium assay; VEGF mRNA and VEGF C mRNA and their protein expressions in CNE-2 cells were detected by RT-PCR and immunohistochemistry. RE- SULTS： The growth of CNE-2 cells was inhibited by thalido- mide in a dose-dependent manner, and the inhibitive rate was 45.6% after incubation with 60 mg/L thalidomide for 72 h. The VEGF mRNA and VEGF-C mRNA expression levels in CNE-2 cells were all reduced after the treatment of thalidomide, especially VEGF mRNA. Their protein expression levels were consistent with their mRNA expression levels. CONCLUSION： Thalidomide maybe inhibit the growth of CNE 2 cells by down- regulating VEGF expression. Chin J Cancer Prey Treat ,2008,15（22） ： 1706-- 1709