HPLC测定细胞中阿霉素及其在筛选耐药逆转剂中的应用

A Method For The Quantitation Of The Adriamycin In Tumour Cell By HPLC And Its Application To Seleting Reverse Agent Of Medicine-Resistant

阿霉素（简称ADM）是肿瘤化疗中常用的药物。本文介绍一种用高效液相色谱（HPLC）测定耐药细胞中ADM的方法，该方法处理简单、灵敏度高，仪器的最低检出量为8．621pmol，在20～344．8pmol范围内呈现良好的浓度一峰高线性关系，并利用该方法时行ADM耐药逆转剂的筛选研究，认为潘生丁、环抱菌素A可作为ADM耐药的逆转剂。

Adriamycin(be called ADM) is a generally medicine in chemistry treatment of tumour. This paper reported the method by using HPLC tO analyze Adriamcin in themedicine - resistant cell. This method is simple, and its sensitivity is high. The minimaldetectable concentratiom of this method is 8621pmol. A good linear correlation betweenconcentration and peak height could be obtained within a range of 20 ~ 344. 8pmol. Westudy on selecting reverse agent of medicine - resistant of ADM by Using this method.Tentatively consider that Dipyridarnolc, Cyclosporin a regard as reverse agent of medicine- resistant of ADM.