野生大豆(Glycine soja)C2H2型锌指蛋白基因的克隆与序列分析

Cloning and Sequence Analysis of a C2H2 type Zinc Finger Protein Gene from Glycine soja

根据大豆SCOF-1的CDs区设计特异引物,通过PCR和RT-PCR从野生大豆01-197中克隆到含两个锌指的C2H2型锌指蛋白基因,命名为GjC2H2,GenBank登录号为FJ172776。对其进行核苷酸和氨基酸同源性分析及系统发生分析,结果表明:该基因内部无内含子,长度为702bp,分子量为25.13KD,与其他锌指蛋白基因有很高的同源性,推测其与植物抗逆性有关。

Specific primers were designed according to the soybean SCOF- I of CDs from the GenBank, C2H2- type Zinc finger protein gene containing two zinc finger, named GjC2H2 was cloned through the PCR and RT-PCR from the wild soybean 01-197, and the GenBank accession number was FJ172776. Homogenesis and phylogeny analysis of nucleotide, amino acid sequence showed that there was no intron in the gene, the length of the gene was 702 bps, molecular weight was 25.13 KD, and the gene and other zinc finger protein gene had a high homology. It is presumed that the gene GjC2H2 is related with stress tolerance in plants.