大豆皂醇抗结肠癌作用的研究

Anti-Colon Tumor Effect of Soyasapogenols

大豆皂甙通过促进肿瘤细胞凋亡可抑制多种肿瘤细胞的增殖。采用C18反相柱层析法从大豆胚轴提取大豆皂甙,再将其部分水解成其甙元大豆皂醇,研究其抗结肠癌细胞增殖作用。采用MTT比色法观察大豆皂醇对结肠癌HT-29细胞增殖的影响,采用TUNEL染色法检测其对HT-29细胞凋亡的影响。结果表明:大豆皂醇在40-160mg.L-1范围内可时间和浓度依赖性地抑制结肠癌细胞增殖和诱导细胞凋亡。用80 mg.L-1大豆皂醇A和B作用结肠癌细胞72 h时,细胞生长抑制率分别为(48.7±1.3)%和(42.6±2.0)%;其对肿瘤细胞凋亡率分别为(37.0±1.2)%和(29.5±0.7)%。提示,大豆皂醇可通过诱导细胞凋亡发挥抗结肠癌作用。

soyasaponins have been observed to limit many kind of tumor cell proliferation by inducing apoptosis. Soyasaponins were isolated from soybean hypocotyl with C,s reversed phase column chromatography,then soyasapogenols were prepared by hydrolysis of soyasaponins and their inhibitory effect on proliferation of colon carcinoma cell was investigated. MTT assay was used to examine the proliferation of HT-29 cell,TUNEL method was used for analysis of cell apoptosis. The resuits showed that soyasapogenols inhibited the proliferation and induced apoptosis of colon carcinoma cells in a time- and concentrationdependent manner in the concentration of 40-160 mg · L^-1. The inhibitory ratio of soyasapogenol A and B on cell proliferation was （48.7 ± 1.3 ）% and（42.6 ± 2.0）%, respectively;the apoptosis ratio on carcinoma cell was （37.0 ± 1.2 ） % and （29.5 ± 0.7） %, respectively. It is suggested that soyasapogenols could exert an anti-colon cancer effect via induction of apoptosis.