补硒对非酒精性脂肪性肝炎大鼠肝脏抗氧化能力的影响

Effect of selenium supply on anti-oxidative capacity in non-alcoholic steatohepatitis rats

目的观察补硒对高脂饮食诱导的非酒精性脂肪性肝炎(NASH)大鼠肝脏抗氧化能力的影响,探讨其治疗脂肪肝的机制。方法24只健康雄性Wistar大鼠随机分为3组,即正常组,模型组和补硒组,每组8只。补硒组在高脂饮食8周后给予亚硒酸钠灌胃干预,同时模型组和正常组分别给予等量的生活饮用水灌胃,13周末处死各组大鼠。HE染色观察肝脏病理组织学改变,测定肝组织TC、TG、丙二醛(MDA)、超氧化物歧化酶(SOD)活性以及谷胱甘肽过氧化物酶(GSH-PX)和硫氧还蛋白还原酶(TrxR)的活性。结果13周末时,与正常组相比,模型组大鼠炎症活动度计分明显升高,肝组织TC、TG、MDA升高,而SOD、GSH-PX、TrxR降低。与模型组相比,补硒组肝脏脂肪变性程度明显改善,肝组织TC、TG、MDA明显降低,SOD、GSH-PX、TrxR则明显升高,差异有统计学意义(P<0.05)。结论补硒能通过对抗氧化应激和脂质过氧化损伤,有效地治疗大鼠非酒精性脂肪性肝炎。

Objective To explore the effect of selenium supply on anti-oxidative capacity in non-alcoholic steatohepatitis rats and its mechanism. Methods Twenty-four male Wistar rats were randomly divided into 3 groups： normal group （ n = 8 ）, model group （ n = 8 ） and selenium group（n = 8 ）. The rats were fed with standard diet in normal group and with high-fat diet in model group and selenium group. After eight weeks, the rats were garaged with sodium selenite（ 8 μg/ml, once a day）in selenium group, while the same volume of life drinking water was given in normal group and model group, respectively. At the end of week 13 after feeding, the rats were executed. The pathological changes were observed, and the levels of liver TC,TG, MDA, SOD, GSH-PX and TrxR were determined. Results At the end of week 13, rats in model group developed hyperlipidemia, but hepatosteatosis in selenium group was obviously improved. Compared with normal group, the levels of liver TC, TG, MDA increased in model group, while SOD, GSH-PX, TrxR decreased. Compared with model group, the levels of liver TC, TG, MDA were lower in selenium group, while SOD, GSH-PX, TrxR were higher. Conclusion Selenium supply can effectively treat non-alcoholic steatohepatitis by anti-oxidative stress and lipid peroxidation damage in liver.