氩激光诱导的大鼠脉络膜新生血管模型研究

Establishment of argon laser-induced choroidal neovascularization model in rats

目的探讨应用氩激光诱导棕色挪威(Brown Norway,BN)大鼠脉络膜新生血管(choroidal neovascularization,CNV)模型建立的可行性,为明确CNV的发生机制及防治研究奠定基础。方法雄性BN大鼠20只(40只眼)随机分为4组,每组5只,每只鼠一只眼作为实验眼,另一只眼作为对照眼(空白对照,不进行激光光凝)。用氩激光(波长为647nm)对大鼠实验眼视网膜进行光凝,激光功率、光凝斑直径和曝光时间分别为200mW、100μm及0.04s,每只眼10个光凝斑点。光凝后7,14,21,56d分别随机抽取1组大鼠,实验眼行荧光素眼底血管造影(fundus fluorescein angiography,FFA)检查后处死动物,摘除眼球制作标本,进行光学显微镜检查(LM)和免疫组织化学染色观察。结果FFA、LM和免疫组织化学染色观察均证实,光凝后7d见CNV开始形成,14d逐渐增多,21d达到高峰并能保持稳定,56d CNV有所减少。结论氩激光损伤可以创建BN大鼠脉络膜新生血管模型,成模时间短,成模率高,是一种较为理想的CNV动物模型。

Objective To evaluate the feasibility of argon laser-induced choroidal neovascularization （CNV）model in Brown Norway （BN）rats for exploring a mechanism of CNV and its treatment. Methods Twenty male BN rats were randomly divided into four groups. One eye without any laser photocoagulation was selected as the control eye, and the other eye with laser photocoagulation was as the experimental eye. Each experimental eye received a series of 10 argon laser lesions using slit lamp delivery system at a spot size of 100 μm for 0.04 s with power of 200 mW and the wavelength of 647 nm. The rats in 4 groups were randomly performed fundus fluores- cein angiography （ FFA）, light microscopy （LM） and immunohistochemistry at day 7,14,21,56 after photocoagulation, respectively. Results FFA, LM and immunohistochemical results demonstrated that CNV began to form at day 7 after photocoagulation,increased at day 14,peaked at day 21 and kept stable until day 56. Conclusion The results indicate that argon laser photocoagulation could be used to establish the choroidal neovascularization experimental model in BN rat with a short time and a high successful rate.