肠源性内毒素血症与肝星形细胞在肝纤维化中的作用

Role of intestinal endotoxemia and hepatic stellate cell in hepatic fibrosis

目的研究IETM与α-SMA在肝纤维化中的作用,以进一步了解二者的关系。方法21只Wistar大鼠随机分为对照组(n=6)和肝纤维化组(n=15)。正常对照组饲以正常饮食,肝纤维化组采用复合因子方法饲养大鼠建立肝纤维化模型。饲养4周后,透射电镜下观察肝星形细胞的活化,免疫组化方法观察α-平滑肌肌动蛋白(α-SMA)的表达,并测定血浆中的内毒素含量,同时也测定了丙氨酸转氨酶(ALT)、透明质酸(HA)、丙二醛(MDA)的水平。结果4周末透射电镜下肝纤维化组观察到了HSC的活化,免疫组化法观察到了肝纤维化组α-SMA表达明显高于对照组,血浆中ET、ALT、HA、MDA的含量肝纤维化组明显高于对照组。结论肝纤维化发生时有肠源性内毒素血症的发生和肝星形细胞的活化,二者之间可能存在一定关联。

Objective To investigate the role of intestinal endotoxemia（IETM） and hepatic stellate cells （HSC）in hepatic fibrosis, and to further explore the relationship of IETM and HSC. Methods All rats were divided into two groups ： normal control group （n = 6） and hepatic fibrosis group（ n = 15 ）. The rats were fed with normal diet in control group and complex diet （including CC14, alcohol, etc） in hepatic fibrosis group. After 4 weeks, the rats were executed. The activation of HSC was observed with transmission electron microscope （TEM）, and the expression of α-SMA in hepatic tissues was examined by immunohistochemical techniques. The changes of plasma endotoxine,ALT, HA, MDA were detected. Results The activation of HSC was observed at the end of 4th week with transmission electron microscope. Immunohistochemical results showed that α-SMA expression in hepatic fibrosis group was higher than in normal control group. Plasma ET, ALT, HA, MDA levels in hepatic fibrosis group were significantly higher than in normal control group. Conclusion IETM and the activation of HSC may occur in hepatic fibrosis.