DYS542在山西汉族人群中的多态性分布

Polymorphism of DYS542 in population of Han ethnicity in Shanxi province in China

目的对DYS542基因座的基因多态性和法医学应用进行研究。方法随机抽取102例山西汉族男性无关个体静脉血,进行聚合酶链反应(PCR)扩增,银染显色。对其筛选的等位基因测序,构建等位基因分型标准物;按照国际法医遗传学会DNA委员会(ISFG)原则命名各等位基因。结果山西地区汉族男性DYS542基因座观察到14,15,16,17,18共5个等位基因,基因频率分别为0.1068,0.4078,0.3495,0.1262,0.0097。该基因座核心重复单位是ATAG。结论Y染色体基因座DYS542具有较高的遗传多态性,在法医学及人类遗传学方面具有较高应用价值。

Objective To investigate the gene polymorphism of locus DYS542 and its utilization. Methods The venous blood sampies were randomly collected from 102 unrelated males in Shanxi province. Each allele of Y-STR loci was sequenced and the allele ladders were constructed. Alleles of Y-STR locus were nominated according to recommendations of the International Society of Forensic Genetics. Results Five alleles named 14,15,16,17,18 were observed with frequency of 0. 106 8,0. 407 8,0. 349 5,0. 126 2, 0.009 7. The number of simple repeat nucleotide was four and the sequence was ATAG. Conclusion DYS542 has a high level of genetic polymorphism and can be used in forensic science and human genetics research.