不同应激诱导对HELA细胞产生IL-6及leptin水平的影响

Effect of Different Stress Factors on IL-6 and Leptin Expression in HELA Cell Cultures

目的:研究高葡萄糖(HG)、脂多糖(LPS)及双氧水(H2O2)应激对HELA细胞上清中白细胞介素-6(IL-6)及leptin含量的影响。方法:分别给予以上三种炎症因子,建立HELA细胞炎症反应模型,采用放射免疫分析测定不同时间点细胞上清中IL-6及leptin含量的变化。结果:与空白对照组相比,三种刺激物作用6h与24h后,IL-6的含量显著升高,而leptin含量明显下降;且随着作用时间延长变化愈加明显,统计学上差异有显著性(P<0.05)。结论:leptin可能作为一种炎性细胞因子在炎症反应中发挥一定的作用。

Objective To study the effect of three stress factors high glucose （HG）, lipepolysaccharide （LPS） and hydrogen peroxide （ H2O2） on the expression of culture supernatant IL - 6 （ IL - 6） and leptin contents of HELA cell line. Methods HELA cell culture models of severe inflammatory repsonse syndrome were prepared with cultures treated with 50mmol/L glucose （ HG）, 41μg/ ml LPS and 100μmol/L H2O2 respectively and supematant contents of IL -6 and leptin were measured with RIA at 1h, 6h and 24h. Results Generally speaking, the culture supenatant contents of IL - 6 gradually increased and leptin contents gradually decreused with significant differences from those in cultures not treated with either stress factor at 6h and 12h （P 〈 0.05）. Conclusion Leptin as a possible anti - inflammatory cytokine might plays an important protective role in severe inflammatory response.