依普利酮对阿霉素诱导心衰大鼠TNF-α的影响

Effect of Eplerenone on Serum TNF-α Levels in Adriamycin Induced Heart Failure Male Rat Models

目的:探讨依普利酮对阿霉素诱导心衰大鼠血清TNF-α的影响及其治疗心衰的可能机制。方法:50只雄性SD大鼠随机挑选40只腹腔注射阿霉素制做心衰模型,其余10只作为正常对照组。6周后将存活的20只模型鼠随机分为2组:心力衰竭+依普利酮治疗组(n=10),给予依普利酮200mg/(kg.d)灌胃,心力衰竭组(n=10)与正常对照组大鼠(n=10)给与安慰剂(生理盐水)灌胃,12周后观测一般变化,测定大鼠心功能及血清TNF-α含量。结果:心衰组血流动力学指标与正常组相比有显著性差异(P<0.05)。治疗组血流动力学指标与心衰组相比有显著性差异(P<0.05)。心衰组TNF-α水平与正常组相比明显升高(P<0.05)。治疗组TNF-α水平与心衰组相比明显下降,有显著性差异(P<0.05)。结论:依普利酮可以降低心衰大鼠血清TNF-α的含量。

Objective To investigate the effect of eplerenone on serum TNF - α levels in adriamyein induced heart failure male rat models. Methods Fourty male rat models of adriamyein - induced heart failure were prepared with weekly intraperitoneal injection of adriarnycin （4/mg/kg） for six weeks. Twenty surviving models were randomly divided into two groups： （1）eplerenone -treated group, n = 10, treated with gavage of eplerenone 200mg/kg/d for 12 weeks （2） non - treated group n = 10. All the surriving models （group （1） n =8, group （2） n =6） were sacrificed after 12 weeks with left ventricular hemodynamie function parameters tested and sertum TNF - α levels measured. Ten male rats without adriamycin administration served as controls. Results Left ventricular hemodynamic parameters in the non - treated group were significantly worse than those in controls （ P 〈 0.05 ）. The parameters in the eplerenone treated group were significantly better than those in the non - treated group （ P 〈 0.05）. The serum TNF - α levels in the non - treated group were significantly higher than those in controls （ P 〈 0.05）. TNF - α levels in the eplerenone group were significantly lower than those in the non - treated group （ P 〈 0.05 ）. Conclusion Eplerenone could reduce the serum TNF - α levels in the rat models of heart failure.