妊高征患者外周血PMBC培养上清液中IL-4和IFN-γ检测的临床意义

Clinical Significance of Detection of Peripheral Blood Mononuclear Cell(PBMC) Culture Supernatant Contents of IL-4 and IFN-γ in Patients with Pregnancy Induced Hypertension Syndrome

目的:探讨对妊高征患者进行IFN-γ、IL-4检测的临床意义。方法:观察不同病情妊高征孕妇与正常妊娠孕妇外周血培养上清单个核细胞(PBMC)中白介素-4(IL-4)和γ-干扰素(IFN-γ)的含量情况,计算IL-4/IFN-γ的比值。结果:与正常妊娠组比较,IL-4轻度妊高征组略有降低,但不明显(P>0.05),中度妊高征组与中度妊高征组则非常明显(分别为P<0.05、P<0.01);IFN-γ轻度妊高征组略有升高,但不明显(P>0.05),中度妊高征组与重度妊高征组比较则非常明显(P<0.01)。妊高征孕妇轻、中、重度组外周血培养上清PBMC中IL-4/IFN-γ的比值均明显低于正常妊娠组(P<0.05或P<0.01);并且随着病情加重,IL-4/IFN-γ的比值与正常妊娠组的差别越明显。结论:妊高征孕妇体内存在Th1/Th2细胞因子失衡现象;并且Th1/Th2细胞因子失衡程度与妊高征的病情密切相关。临床可以通过检测孕妇外周血IL-4和IFN-γ含量,并计算IL-4/IFN-γ的比值,作为评价妊高征患者病情严重程度和估计预后的指标。

Objective To investigate the clinical significance of detection of PBMC culture supematant contents of IL - 4 and IFN -γ in patients with pregnancy induced hypertension （PIH）. Methods The levels of IL- 4 and γ -interferon （INF -γ） in the PBMC culture supernatant were determined with ELISA and IL - 4/IFN - γ ratio was calculated in 62 pregnant women with PIH and 30 controls. Results The supernatant contents of IL -4 mild patients with mild PIH was slightly lower, but not significantly than those in controls （ P 〉 0.05 ）, while in patients with moderate and severe PIH （ n = 17, n = 15） the IL - 4 contents lower than those in controis were significantly （P 〈0.05 and P 〈0.01 respectively）. However, the reverse was exactly true with IFN -γcontents. IFN -γ in mild PIH group increased only slightly, without significant difference （ P 〉 0.05）, was significantly lower in patients with moderate severe PIH than that of the controls （P 〈 0.05 or P 〈 0.01 ）. Conclusion Imbalance in the T - helper1/helper2 （Th1/Th2） ratio in peripheral blood was present in patients with PIH. Clinically, detection of IL-4, IFN-γ/and IL-4/IFN-γ ratio in the peripheral blood mononuclear cell culture supernatant from patients with PIH could be taken as a predictive marker for disease severity and prognosis.