胰岛素及丹参对体外培养成纤维细胞影响的实验研究

THE EXPERIMENTAL STUDY OF THE EFFECT OF INSULIN AND DANSHEN ON CULTURE OF FIBROBLAST IN VITRO

为了探讨胰岛素、丹参在伤口愈合过程中对胶原合成与分解代谢的影响，应用人胚皮肤培养成纤维细胞，分设胰岛素组、丹参组及对照组，培养２，４和６天，观察细胞的生长情况。成纤维细胞生长曲线显示：胰岛素组高于对照组，对照组高于丹参组。细胞核分裂率三组分别为１９．６０‰，２．５０‰和３．７７‰。扫描电镜观察发现胰岛素组成纤维细胞周围及表面有大量纤维状物，丹参组成纤维细胞表面光滑，仅有少量纤维状物。认为，胰岛素对成纤维细胞增殖和胶原合成有促进作用。

To investigate the effect of Insulin and Danshen on anabolism and catabolism of collagen during the healing of wound, fibroblast which was cultured from human embryonic skin were divided into 3 groups: Insulin group, Danshen group and contral group. Each group was cultured for 2,4 and 6 days, then the growth curve was established respectively. Results: ①the growth curve showedInsulin group> control group> Danshen group. ②the rate of cell division in 3 groups was 19.6‰, 2.5‰, 3.77‰ respectively. ③the electron microscopic scanning showed that there was much of fibroid tissue surrounding the fibroblast cell in insulin group, but there was little fibroid tissue in Danshen group. The conclusion showed that insulin can accelerates proliferation of fibroblast and synthesis of collagen, but the effect of denshen was just on the contrary.