人类乳头状瘤病毒与肺癌的病因关系研究

The etiologic relationship of HPV infection to the development primary squamous cell carcinoma of lung

目的：研究人类乳头状瘤病毒（ＨＰＶ）与肺鳞癌发生的病因关系。方法：采用聚合酶链反应（ＰＣＲ）技术检测石蜡包埋肺鳞癌组织标本５０份，肿瘤组织旁鳞状化生上皮标本３０份，正常支气管粘膜３０份中的ＨＰＶＤＮＡ。结果：上述三种标本中，ＨＰＶＤＮＡ的检出率分别为２６％、３７％和１０％，其中以ＨＰＶ１６型所占比例最高。结论：结果表明ＨＰＶ感染与肺鳞癌的发生有一定的联系，鳞状上皮化生似可视为癌前病变。

Objective:To study the etiologic relationship of HPV infection to the development of primary squamous cell carcinoma of lung.Methods:50 cases of formalinfixed paraffinembedded tissues of carcinoma of the lung,30 samples of areas of squamous epithelium metaplasia nextto totumors,and 30 cases of normal bronchial mucosa were studied for the presence of human papillomavirus (HPV) DNA by polymerase chain reaction (PCR).Results:In these samples,the positive detected rates of HPV DNA were 26%, 37% and 10%, Respectively. HPV 16 was the highest.The detected rates of HPV 6/11 and HPV 16 in carcinoma and squamous epithelium metaplasia had not significant differnce (P>005).Conculusion:HPV infection might play an important role in the devolopment of carcinoma of lung and squamous epithelium metaplasia of bronchus could be regarded as a precancer lesion.PCR has a higher sensitivity compared with biotinylated DNA probes and hybridization techniques.