鼠疫菌pYC质粒标识基因在动物及蚤检测中的应用

Application of Yersinia pestis Label Gene of Plasmid pYC in Detecting Animals and Fleas

目的评价云南鼠疫菌pYC质粒标识基因的特异性及在鼠疫监测中的应用。方法应用pYC质粒标识基因引物yd1,yd2并以鼠疫菌特异性基因pla,fra作为对照进行PCR实验,观察实验室感染标本和现场样品的检出情况。结果共检测实验感染动物脏器8份,均为阳性。检测现场收集的黄胸鼠脏器22份,阳性6份,阳性率27.27%。检测感染的蚤类67份,阳性13份,阳性率19.40%,试验引物yd1,yd2与对照引物pla,fraPCR检测结果其特异性和敏感性均一致。结论将yd1,yd2引物,联合pla,fra引物,建立鼠疫PCR快速诊断方法,应用于云南、广西、贵州判断鼠疫菌、监测鼠疫信息,具有重要的实际意义。

Objective To observe the specificity and application of the label gene of Yersinia pestis plasmid pYC in surveillance of plague. Methods With primers yd1, yd2 and comparing primersfra and pla from the specific genes of Yersinia pestis plasmids, the infectious samples from testing and spot were detected by PCR. Results Eight infectious samples by testing all were positive. There were 6 positive samples in 22 sampies collected from spot with the positive rate of 27.27%. There were 13 positive samples in 67 infectious fleas, the positive rate was 19.40%. The specificity and sensitivity were consistent about testing primers and comparing primers. Conclusions Combining the primers yd1, yd2 and pla, fla can establish a rapid PCR method of plague. It will be of practical importance in diagnosis and surveillance of plague in Yunnan, Guangxi and Guizhou provinces.